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Anti cGMP Mouse Monoclonal Antibody

cGMP [26001-1]
[30 µL]

Catalog Number:  26001-1
Gene Symbol:  
Description:   Anti-cGMP Mouse Monoclonal Antibody
Background:   cGMP is a ubiquitous second messenger mediating cellular responses to various exogenous and endogenous signaling molecules. cGMP regulates physiological processes by activating protein kinases, gating specific ion channels, and modulating cellular cyclic
Immunogen:   cGMP
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA 1:1000-1:5000, WB 1:1000-1:2000
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Typical Standard Curves  
    Sensitivity 
 

Acetylated Version
Mean OD for Bo = 0.536 ± 0.010
Mean OD for Standard #6 = 0.403 ±0.009
Delta Optical Density (0-0.08 pmol/mL) = 0.133
2 SD's of the Zero Standard = 0.020
Sensitivity =(0.020/0.133)*0.08 pmol/mL=12 fmol/mL

Publications:
1.  Sulfhydryl-dependent dimerization of soluble guanylyl cyclase modulates the relaxation of porcine pulmonary arteries to nitric oxide
    Pflugers Arch. 2013 Feb;465(2):333-41
2.  Pressure-overload-induced subcellular relocalization/oxidation of soluble guanylyl cyclase in the heart modulates enzyme stimulation
    Circ Res. 2012 Jan 20;110(2):295-303
3.  Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity
    Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17681-6
4.  Role of sulfhydryl-dependent dimerization of soluble guanylyl cyclase in relaxation of porcine coronary artery to nitric oxide
    Cardiovasc Res. 2011 Jun 1;90(3):565-72
5.  Molt-inhibiting hormone from Chinese mitten crab (Eriocheir sinensis): Cloning, tissue expression and effects of recombinant peptide on ecdysteroid secretion of YOs
    Gen Comp Endocrinol. 2011 Sep 15;173(3):467-74
6.  Inhibition of Striatal Soluble Guanylyl Cyclase-cGMP Signaling Reverses Basal Ganglia Dysfunction and Akinesia in Experimental Parkinsonism
    PLoS One. 2011;6(11):e27187
7.  Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish
    Comp Biochem Physiol B Biochem Mol Biol. 2011 Jan;158(1):46-55
8.  Feedback control through cGMP-dependent protein kinase contributes to differential regulation and compartmentation of cGMP in rat cardiac myocytes
    Circ Res. 2010 Nov 12;107(10):1232-40
9.  The Soluble Guanylate Cyclase Activator BAY 58-2667 Protects against Morbidity and Mortality in Endotoxic Shock by Recoupling Organ Systems
    PLoS One. 2013 Aug 28;8(8):e72155
10.  Stimulation of guanylyl cyclase-D by bicarbonate
    Biochemistry. 2009 May 26;48(20):4417-22
11.  Volume overload induces differential spatiotemporal regulation of myocardial soluble guanylyl cyclase in eccentric hypertrophy and heart failure
    Journal of Molecular and Cellular Cardiology Volume 60, July 2013, Pages 72–83
12.  Defining Specificity Determinants of Cyclic GMP-Mediated Gustatory Sensory Transduction in Caenorhabditis elegans
    genetics.113.152660v1 194/4/885
13.  The role of nitric oxide signaling in food intake; insights from the inner mitochondrial membrane peptidase 2 mutant mice
    Redox Biology Volume 1, Issue 1, 2013, Pages 498–507
14.  Cross regulation between cGMP-dependent protein kinase and Akt in vasodilatation of porcine pulmonary artery
    Journal of Cardiovascular Pharmacology: November 2014 - Volume 64 - Issue 5 - p 452-459
15.  Genetic ablation of solute carrier family 7a3a leads to hepatic steatosis in zebrafish during fasting
    Hepatology Volume 60, Issue 6, pages 1929–1941, December 2014

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