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Anti Rac1 Mouse Monoclonal Antibody

Gα13 [26004]
[100 µL]

Catalog Number:  26004
Gene Symbol:  Gna13
Description:   Anti-Gα13 Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Immunogen:   Recombinant full length Gα13 protein
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA 1:1000-1:5000, WB 1:1000-1:2000
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  13 monoclonal antibody recognizes Gα13 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of Gα13 proteins. Gα13, Gα12 or Gαq plasmid DNAs were transiently transfected into CHO cells (endogenous Gα13 protein was down-regualted by Gα13 siRNA). Whole-cell lysates were used for western blots.

Publications:
1.  Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization
    J Biol Chem. 2011 Sep 2;286(35):31055-61
2.  Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2
    Br J Pharmacol. 2011 Feb;162(3):733-48
3.  Gα12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation
    Carcinogenesis (2010)
4.  Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency
    J Biol Chem. 2009 Oct 2;284(40):27409-15

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