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Anti IDH1(R132S) Mouse Monoclonal Antibody

IDH1 (R132S)
Catalog Number: 26160
Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD
Description: Anti-IDH1 (R132S) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenases
catalyze the oxidative decarboxylation of
isocitrate to 2-oxoglutarate. Mutations affecting
Arg-132 are tissue-specific, and suggest that this
residue plays a unique role in the development
of high-grade gliomas. Mutations of Arg-132 to
Cys, His, Leu or Ser abolish magnesium binding
and abolish the conversion of isocitrate to
alpha-ketoglutarate. Instead, alpha-ketoglutarate
is converted to R-2-hydroxyglutarate. Elevated
levels of R-2-hydroxyglutarate are correlated
with an elevated risk of malignant brain tumors.
Immunogen: A synthetic peptide from the
internal region of IDH1 which includes the
mutation of R132S, human origin.
Tested applications: ELISA, WB, IHC
Recommended dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration: 1 mg/ml
Host: Mouse
Clonality: Monoclonal
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes IDH1 (R132S)
mutated prteein, but not wild-type IDH1 of
vertebrates.
Storage Conditions: Store at -20°C. Avoid

freeze / thaw cycles

Western blot analysis of recombinant IDH1
(R132S) and wild-type proteins. Purified
His-tagged IDH1 (R132S) protein (lane 2) and
wild-type protein (lane 1) were blotted with
anti-IDH1 (R132S) mouse antibody (Cat.
#26160).

Immunofluorescence of cells expressing IDH1
proteins with anti-IDH1 (R132S) antibody.
HEK293T cells were transfected with
pCDNA3-GFP-IDH1 (WT) plasmid (left column)
or pCDNA3-GFP-IDH1 (R132S) plasmid (right
column), then fixed and stained with anti-IDH1
(R132S) monoclonal antibody (Cat. #26160).

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